Journal: Journal of Biological Engineering

Article Title: Experimental studies from shake flasks to 3 L stirred tank bioreactor of nutrients and oxygen supply conditions to improve the growth of the avian cell line DuckCelt®-T17

doi: 10.1186/s13036-023-00349-5

Figure Lengend Snippet: Characterization of the DuckCelt®-T17 culture process performed using reference operating conditions from in a 3 L stirred bioreactor. Time evolution of cell growth (solid line) and viability percentage (dotted line) (a), of the concentration of metabolites involved in glutaminolysis (b) and glycolysis (c). Gln: glutamine (solid line); NH 4 + : ammonium (dotted line); Glc: glucose (solid line); Lac: lactate (dotted line). Results are presented as means ± SD (n = 3)

Article Snippet: The cells were cultured in an Applikon 3 L glass bioreactor equipped with a marine impeller of the elephant ears type and connected to an eZ-control device (Getinge, Göteborg, Sweden).

Techniques: Concentration Assay

Journal: Journal of Biological Engineering

Article Title: Experimental studies from shake flasks to 3 L stirred tank bioreactor of nutrients and oxygen supply conditions to improve the growth of the avian cell line DuckCelt®-T17

doi: 10.1186/s13036-023-00349-5

Figure Lengend Snippet: Effect of dO 2 on cell growth (a), viability (b) and growth kinetic parameters (c) for DuckCelt®-T17 cells cultivated in a 3 L bioreactor. The cells were grown at 10% (grey line), 30% (blue line) or 50% (green line) dO 2 . Results are presented as means ± SD (n = 3 for 30% and n = 2 for 10% dO 2 ). The data for 50% dO 2 (n = 3) originate from the reference process experiments

Article Snippet: The cells were cultured in an Applikon 3 L glass bioreactor equipped with a marine impeller of the elephant ears type and connected to an eZ-control device (Getinge, Göteborg, Sweden).

Techniques:

Journal: Journal of Biological Engineering

Article Title: Experimental studies from shake flasks to 3 L stirred tank bioreactor of nutrients and oxygen supply conditions to improve the growth of the avian cell line DuckCelt®-T17

doi: 10.1186/s13036-023-00349-5

Figure Lengend Snippet: Scale-up in a 3 L bioreactor of the DuckCelt®-T17 culture using batch (glutamine and glutamax), fed-batch (glutamax + SFM) or perfusion processes. Time evolution of cell growth (solid line) (a), viability percentage (dotted line) (b) and waste product concentration (ammonium in solid line and lactate in dotted line) (c). Comparison of metabolic profiles at day 9 between the culture permeate and the bioreactor culture medium (d). Results are presented as means ± SD (n = 3 for strategies A and M, n = 2 for strategy B, n = 1 for perfusion assay)

Article Snippet: The cells were cultured in an Applikon 3 L glass bioreactor equipped with a marine impeller of the elephant ears type and connected to an eZ-control device (Getinge, Göteborg, Sweden).

Techniques: Concentration Assay

Journal: Microbial Cell Factories

Article Title: Development of a fed-batch process for a recombinant Pichia pastoris Δ och1 strain expressing a plant peroxidase

doi: 10.1186/s12934-014-0183-3

Figure Lengend Snippet: Bioreactor cultivation DoE6. Black line, carbon dioxide evolution rate (CER) depicting metabolic activity; black dot, specific substrate uptake rate (q s ). All cultivations ran in four phases: 1, batch on glycerol; 2, non-induced fed-batch on glycerol; 3, methanol adaptation pulse; 4, fed-batch on methanol.

Article Snippet: Batch cultivations were carried out in a 3 L working volume glass bioreactor (Infors, Switzerland).

Techniques: Activity Assay